Journal:

Article Title: YRRL motifs in the cytoplasmic domain of the thrombopoietin receptor regulate receptor internalization and degradation

doi: 10.1182/blood-2008-01-134049

Figure Lengend Snippet: c-Mpl internalization is reduced in AP2α2-depleted cells. BaF-Mpl cells were transfected with siRNA specific to the AP2α2 subunit, which is essential for the function of AP2. (A) AP2α2 depletion was analyzed by Western blot and was maximal 48 hours after transfection (approximately 75% depletion). (B) Tpo-stimulated c-Mpl internalization was then determined by flow cytometry in AP2α2-depleted BaF-Mpl cells 48 hours after transfection (**P < .01). Error bars represent SD.

Article Snippet: Monoclonal mouse anti-AP2α2 antibody was purchased from BD Biosciences (La Jolla, CA).

Techniques: Transfection, Western Blot, Flow Cytometry

Journal:

Article Title: YRRL motifs in the cytoplasmic domain of the thrombopoietin receptor regulate receptor internalization and degradation

doi: 10.1182/blood-2008-01-134049

Figure Lengend Snippet: The Y78F mutation leads to increased Tpo-mediated proliferation and signaling. (A) MTT proliferation assays were performed on c-Mpl wild-type–, Y8F-, Y78F-, and Y8+78F-expressing cells. Data points represent the mean of 3 independent experiments using 3 different c-Mpl–expressing clones (**P < .01). Error bars represent SE. (B) Levels of phosphorylated Jak2, STAT5, AKT, and ERK1/2 in response to 50 ng/mL Tpo were determined by Western blot analyses. (C) Tpo-stimulated activation of Jak2, STAT5, AKT, and ERK1/2 in wild-type c-Mpl–expressing cells treated with control or AP2α2 siRNA. Data are representative of 3 independent experiments.

Article Snippet: Monoclonal mouse anti-AP2α2 antibody was purchased from BD Biosciences (La Jolla, CA).

Techniques: Mutagenesis, Expressing, Clone Assay, Western Blot, Activation Assay